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การประชุมวิชาการการศึกษาพยาธิสภาพของเนื้อเยื่อที่มีลักษณะกล้ามเนื้อขุ่นขาวชนิดต่าง ๆ ในการเลี้ยงกุ้งขาวแวนนาไม (2010)ผู้แต่ง:Dr.Chalor Limsuwan, Associate Professor, Dr.Niti Chuchird, Associate Professor, สาธิต ประเสริฐศรี, สุธี วงศ์มณีประทีป, แก้วตา ลิ้มเฮง, ปัทมา วิริยพัฒนทรัพย์, ทิพวรรณ ไกรวิลาศ, เกศินี หลายสุทธิสาร, การประชุมวิชาการ: |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Efficacy of Calcium Hypochlorite on the Prevalence of Microsporidiosis (Thelohania) in Pond-Reared Litopenaeus vannamei) ผู้เขียน:ดร.ชลอ ลิ้มสุวรรณ, รองศาสตราจารย์, ดร.นิติ ชูเชิด, รองศาสตราจารย์, เกศินี หลายสุทธิสาร สื่อสิ่งพิมพ์:pdf AbstractThe efficacy of calcium hypochlorite for controlling microsporidian (Thelohania or Agmasoma) infection by treating the water before stocking Pacific white shrimp, Litopenaeus vannamei, postlarvae (PL) was studied in intensively cultured ponds. Five ponds (of 4 rai each) were used as the treatment group in which calcium hypochlorite at a concentration of 18 mg/l was added into the ponds while all the aerators were operating. In the control group, no calcium hypochlorite was used. PL10 were stocked at a density of 80 PL/m2 and shrimp were fed with commercial pelleted feed throughout the 120-day culture period. Results showed that the prevalence of microsporidian (Thelohania, Agmasoma) infection of shrimp in the treatment group was significantly lower than that of the control group (P<0.05). At day 50, the average highest percentage of microsporidian infection in the treatment ponds was 5.40% compared with 25.16% in the control ponds. The infected shrimp gradually died off or were eaten by the healthy shrimp so that after harvesting only a few infected shrimp were found. In conclusion, in shrimp culture areas where microsporidian outbreaks previously occurred, calcium hypochlorite at the concentration of 18 mg/l should be used for water treatment before stocking the PL. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Observation of Two Viruses in Cultured Giant Freshwater Prawns (Macrobrachium rosenbergii de Man) in Thailand) ผู้เขียน:เกศินี หลายสุทธิสาร, Chalor Limsuwan1, ดร.นิติ ชูเชิด, รองศาสตราจารย์, Timothy William Flegel สื่อสิ่งพิมพ์:pdf Abstract Two viruses in the hepatopancreas (HP) of Macrobrachium rosenbergii postlavae (PL) and adult prawns are described. Investigations were carried out by light microscopic examination of HP tissue squashes stained with 0.05 % malachite green and histological sections stained with hematoxylin and eosin (H&E), as well as by transmission electron microscopy (TEM). Histologically, the first pathogen was a baculo-like virus (BLV), recognized by the presence of enlarged nuclei with marginated nucleoli and eosinophilic inclusions that completely filled the nucleus of the HP tubular epithelium. In contrast, the malachite green-stained HP squashes did not show any marginated nucleoli. TEM of the infected HPs revealed the presence of BLV particles of approximately 250-300 nm in enlarged nuclei. The second virus was a hepatopancreatic parvo-like virus (HPLV). Spherical intranuclear inclusions were easily observed in the HP tubular epithelium in both fresh malachite green-stained tissue squashes and H&E- stained histological sections. TEM of infected HPs revealed intranuclear inclusions containing small virus-like particles of approximately 20-24 nm in diameter. PL (n=200) with percentage infection rates of 20% with the BLV, 50% with the HPLV and 10% with dual infection were stocked into earthen ponds at a density of 60 000 PL/1600 m2. After rearing for 60 d, the prawns were transferred into earthen ponds at a density of 10 000 prawns/1600 m2. Sixty days later, (120-day-old) prawns were transferred into earthen ponds at a density of 5000 prawns/ 1600 m2 and reared until harvesting at day 180. The prawns were sampled and divided into small and large-sized prawns at 60, 120, 150 and 180 d for histological studies to determine the viral infections. Results showed that at 120 d, the BLV infection in the small-sized prawns (12.5%) was higher than that of the large-sized prawns (5%). Dual infection with both viruses was 5.0% in small-sized prawns and 2.5% in large-sized prawns. However, at day 150, the BLV pathogen was not observed in either size group. In contrast, the HPLV infection was found in prawns throughout the culture period. The percentage infection rates in small-sized prawns at days 120, 150 and 180 were 22.5, 15.0 and 12.5%, respectively, similar to the infection rates for large-sized prawns of 20.0, 12.5 and 12.5%, respectively. The percentage survival rate and yield from both ponds at harvest were 67-68% and 197-199 kg/1600 m2, respectively. These results indicated that the infection rates of these two viruses did not increase during the culture period and that the virus infections might have a negative effect on production. |
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การประชุมวิชาการผลของยาออกซิเตตร้าซัยคลิน และยาซัลฟาไดเม็ทท็อกซีนร่วมกับไตรเม็ทโธพริมในการยับยั้งการเจริญ และฆ่าเชื้อวิบริโอ ที่แยกได้จากกุ้งขาวแวนนาไมที่มีอาการโรคขี้ขาว (2011)ผู้แต่ง:กอบทรัพย์ ผลเจริญ, Dr.Chalor Limsuwan, Associate Professor, Dr.Niti Chuchird, Associate Professor, Dr.Watchariya Purivirojkul, Associate Professor, พุทธรัตน์ เบ้าประเสริฐกุล, เต็มดวง สมศิริ, ฐิติพร หลาวประเสริฐ, สุปราณี อะระอิ , เกศินี หลายสุทธิสาร, การประชุมวิชาการ: |
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